?unique=bba19df)
?unique=bba19df)
Acrylink MPLN
Polynucleotide grafter for ExM
Acrylink MPLN is a specialized reagent engineered for the direct covalent grafting of DNA and RNA into hydrogel matrices for Expansion Microscopy (ExM) and related clearing techniques. Based on a nitrogen mustard–acrylate design, Acrylink MPLN introduces reactive groups that alkylate nucleic acid bases while simultaneously providing an acrylate handle for co-polymerization into the gel.
This unique chemistry ensures efficient retention of nucleic acids during gelation, digestion, and expansion, enabling high-resolution visualization of genomic and transcriptomic structures within biological samples. Unlike probe-only approaches, Acrylink MX allows for the anchoring of endogenous DNA and RNA directly, making it an invaluable tool for studies of chromatin organization, RNA localization, and nucleic acid–protein interactions.
Key Features:
- Direct covalent linking of nucleic acids to the ExM hydrogel
- Compatible with both DNA and RNA in cells and tissues
- Robust retention through digestion and expansion steps
- Optimized for use in Expansion Microscopy and optical clearing workflows
Available Sizes:
- Small – 1 mg
- Medium - 5 mg
- Large - 10 mg
With Acrylink MPLN, researchers can achieve reliable nucleic acid anchoring and unlock nanoscale insights into genome and transcriptome organization using standard fluorescence microscopy.