Acrylink Biotin

Acrylink Biotin is a versatile reagent designed for covalent biotinylation and anchoring of biomolecules in Expansion Microscopy workflows. Built on a multifunctional design, Acrylink Biotin combines:

• an active ester group for efficient coupling to amines on proteins, antibodies, or functianlized nucleic acids
• a polymerizable acrylate moiety for incorporation into the hydrogel network, and
• a biotin tag for subsequent high-affinity detection via streptavidin-based probes.

This triple-function chemistry enables researchers to both anchor biomolecules securely into the gel and retain the ability to flexibly amplify or detect signals post-expansion through biotin-streptavidin interactions.

Key Features:

• Direct biotinylation and hydrogel anchoring in one step
• Robust covalent retention of targets during ExM
• Compatible with streptavidin-based amplification for enhanced sensitivity
• Flexible use in pre- and post-expansion labeling strategies

Available Sizes:

• Small – 1 mg
• Large – 5 mg

With Acrylink Biotin, you gain a powerful tool for anchoring, detecting, and amplifying biomolecular signals in expanded samples, enabling high-resolution insights while maintaining compatibility with standard fluorescence workflows.